Anti-Ki-67 Antibody

Our Anti-Ki-67 primary antibody from PhosphoSolutions is chicken polyclonal. It detects human, mouse, and rat Ki-67 and is total IgY fraction. It is great for use in WB, IHC, ICC.

Product Specifications

Background

Ki-67 is a nuclear protein that is considered to be essential for cellular proliferation since it is expressed only in active phases of the cell cycle (G1, S, G2 and mitosis) but not in resting phase G0 (Scholzen & Gerdes 2000) . Ki-67 antibodies have therefore been widely used as markers of cell growth. Ki-67 has further been identified as a predicative and prognostic marker in breast, lung, cervical and other cancers (Yerushalmi et al., 2010; Folescu et al., 2018; Das et al., 2018) .

Short Description

Our Anti-Ki-67 Antibody primary antibody from Aves Labs is chicken polyclonal. It detects Ki-67 and is total igy fraction. It is great for use in WB, ICC.

Synonyms

Antigen identified by monoclonal antibody Ki 67 antibody, Antigen identified by monoclonal antibody Ki-67 antibody, Antigen KI-67 antibody, Antigen KI67 antibody, Antigen Ki67 antibody, Ki67 antibody, KI67_HUMAN antibody, KIA antibody, Marker of proliferation Ki-67 antibody, MIB 1 antibody, MIB antibody, MKI67 antibody, PPP1R105 antibody, Proliferation marker protein Ki-67 antibody, Proliferation related Ki 67 antigen antibody, Protein phosphatase 1 regulatory subunit 105 antibody, RP11-380J17.2 antibody

Other Statements

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Gene Name

MKI67

NCBI Gene ID

4288

UniProt

P46013

Host

Chicken

Species Reactivity

Human, Mouse, Rat

Reactivity

Human, Mouse, and Rat

Immunogen

Recombinant human ki-67 protein expressed in and purified from E. Coli.

Immunogen Type

Human

Target

Ki-67

Clonality

Polyclonal

Isotype

IgY

Conjugation

Unconjugated

Applications

WB, ICC

Field of Research

Cell Markers Neuronal Markers Proliferating Neurons

Assay Type

Primary Antibody

Assay Protocol

Total IgY fraction

Concentration

Lot Dependent

Format

Total IgY fraction

Form

Total IgY fraction

Buffer

PBS + 10 mM NaN3

Molecular Weight

345-395 kDa

Precautions

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Shipping Conditions

Ice Packs

Storage Conditions

Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.

Shelf Life

After date of receipt, stable for at least 1 year at -20°C.

IHC Dilution

1:1000-1:5000

WB Dilution

1:5000

Specificity

Specific for endogenous levels of the ~345 & 395 kDa Ki-67 isoforms. Additional bands are thought to be breakdown products of these 2 isoforms.

Pre Treatment

Total IgY fraction

Target Description

Ki-67 is a nuclear protein that is considered to be essential for cellular proliferation since it is expressed only in active phases of the cell cycle (G1, S, G2 and mitosis) but not in resting phase G0 (Scholzen & Gerdes 2000). Ki-67 antibodies have therefore been widely used as markers of cell growth. Ki-67 has further been identified as a predicative and prognostic marker in breast, lung, cervical and other cancers (Yerushalmi et al., 2010; Folescu et al., 2018; Das et al., 2018).