Anti-FITC | fluorescein isothiocyanate, HRP-conjugated (monoclonal, Clone NI 239)
Selected Variant
AS18 4215
Size: 200 µg
Product Specifications
Background
FITC is a fluorochrome dye that absorbs ultraviolet or blue light causing molecules to become excited and emit a visible yellow-green light. This emission ceases upon removal of the light causing the excitation. FITC antibody is used to enhance the specific signal from a monoclonal antibody or a secondary antibody conjugated to FITC. The sensitivity of the FITC hapten-anti-hapten system makes it a useful alternative to other systems.
CAS Number
9007-83-4
HS Code
38221900
Host
Mouse
Reactivity
FITC molecule
Immunogen
Highly purified fluorescein isothiocyanate isomer 1
Clonality
Monoclonal
Applications
ELISA (ELISA), Immunocytochemistry (ICC), Immunohistochemistry (IHC) paraffin, Dot blot (Dot), Western blot (WB)
Field of Research
BFP/FITC/GFP/RFP/TRITC/YFP
Dilution
The optimum working dilution should be determined by titration by end user. 1:500 (ELISA), 1:50 to 1:200 (ICC, IHC), 1: 1000 (WB) .
Purity
Purified IgG.1 in PBS pH 7.2, HRP conjugated.
Format
Lyophilized
Reconstitution
For reconstitution add 0.5 ml of sterile water
Precautions
This product has not been tested for cross-reactivity with other fluorescent compounds
Additionnal Information
This product is intended FOR RESEARCH USE ONLY.Peroxidase/IgG protein molar ratio (E/P) approximately 1.7.
Storage Conditions
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Stort time storage at +4°C.
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