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Anti-HA (rabbit polyclonal)

Product Specifications

Background

HA-tag is derived from a human influenza hemagglutinin HA-molecule corresponding to amino acids 96-106 and is used as a general epitope tag in expression vectors.

CAS Number

9007-83-4

HS Code

38221900

Host

Rabbit

Reactivity

HA-tag

Not reactive in

No confirmed exceptions from predicted reactivity are currently known

Immunogen

KLH-conjugated synthetic peptide: YPYDVPDYA

Clonality

Polyclonal

Applications

Western blot (WB), immunoprecipitation (IP)

Field of Research

DIG/HA/HSV/KT3/Myc/S/T7/V5/VSV-G

Dilution

1 : 5000 (WB)

Purity

Affinity purified serum in PBS, pH 7.4.

Format

Lyophilized

Reconstitution

For reconstitution add 100 µl of sterile water

Molecular Weight

Depends on the MW of the protein which is HA-tagged

Precautions

Important note: there is astrongcross reactivity between 25 -20 kDa inArabidopsis thalianawildtype.

References & Citations

Tanigawa et al. (2024) . FYVE1/FREE1 is involved in glutamine-responsive TORC1 activation in plants. iScience. 2024 Aug 26;27 (9) :110814. doi: 10.1016/j.isci.2024.110814. Hwang et al. (2019) Arabidopsis ABF3 and ABF4 Transcription Factors Act with the NF-YC Complex to Regulate SOC1 Expression and Mediate Drought-Accelerated Flowering. Mol Plant. 2019 Apr 1;12 (4) :489-505. doi: 10.1016/j.molp.2019.01.002

Storage Conditions

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

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