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FLIP Antibody : FITC (OAPB00045-FITC)

Product Specifications

Gene Aliases

EWSR2, SIC-1, BDPLT21

Gene ID

2313

Swiss Prot

Q01543

Accession Number

AAC51623

Host

Rabbit

Reactivity

Human

Immunogen

The immunogen for anti-FLIP antibody: antibody was raised against a peptide corresponding to amino acids 191 to 209 at C-terminus of human FLIP form2.

Target

(CT) FLICE inhibitory protein

Clonality

Polyclonal

Conjugation

FITC: Fluorescein Isothiocyanate

Type

Polyclonal Antibody

Applications

ELISA, WB, ICC, IHC-P, IF

Purification

Affinity chromatography purified via peptide column

Concentration

1 mg/ml

Homology

Human

Format

Liquid. Purified antibody supplied in 1x PBS buffer.

Reconstitution

All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil) . Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding.

References & Citations

Thome M, Schneider P, Hofmann K, Fickenscher H, Meinl E, Neipel F, Mattmann C, Burns K, Bodmer JL, Schroter M, Scaffidi C, Krammer PH, Peter ME, Tschopp J. Viral FLICE-inhibitory proteins (FLIPs) prevent apoptosis induced by death receptors. Nature 1997;386:517-521 ; Irmler M, Thome M, Hahne M, Schneider P, Hofmann K, Steiner V, Bodmer JL, Schroter M, Burns K, Mattmann C, Rimoldi D, French LE, Tschopp J. Inhibition of death receptor signals by cellular FLIP. Nature 1997;388:190-195 ; Shu HB, Halpin DR , Goeddel DV. Casper is a FADD- and caspase-related inducer of apoptosis. Immunity 1997;6:751-763 ; Hu S, Vincenz C, Ni J, Gentz R, Dixit VM. I-FLICE, a novel inhibitor of tumor necrosis factor receptor-1- and CD-95-induced apoptosis. J Biol Chem 1997;272:17255-17257

Shipping Conditions

Wet Ice

Specificity

Antibody recognizes human and murine FLIPgamma and FLIPdelta but not FLIPalpha and FLIPbeta.

Applications Notes

DcR2 antibody can be used for detection of DcR2 expression by Western blot at 1 ug/mL. Antibody can also be used for immunocytochemistry starting at 10 ug/mL and Immunohistochemistry starting at 2.5 ug/mL. For immunofluorescence start at 20 ug/mL.

NCBI Gene Symbol

FLIP

NCBI GB Accession Number

FLIP

Host or Source

Rabbit

Protein Name

Friend leukemia integration 1 transcription factor

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