Human Glypican-5/GPC5 ELISA Kit PicoKine®, Carrier-free

Human Glypican-5/GPC5 ELISA Kit PicoKine® (96 Tests). Quantitate Human GPC5 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate ). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Glypican-5 is a protein that in humans is encoded by the GPC5 gene. This gene is mapped to 13q31.3. Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>60</td><td>72</td><td>63</td><td>72</td><td>66</td><td>5.35</td><td>8.1%</td></tr><tr><td>Sample 2</td><td>226</td><td>232</td><td>264</td><td>250</td><td>243</td><td>15.00</td><td>6.1%</td></tr><tr><td>Sample 3</td><td>938</td><td>925</td><td>1033</td><td>1044</td><td>985</td><td>53.83</td><td>5.4%</td></tr></table>*number of samples for each test n=16.

Synonyms

Glypican-5; Secreted glypican-5; GPC5

Gene Name

glypican 5

Gene ID

2262

UniProt

P78333

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: E25-T554

Tissue Specificity

In adult, primarily expressed in the brain. Also detected in fetal brain, lung and liver.

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, serum and plasma (heparin, EDTA, citrate ).

Detection Range

31.2 pg/ml - 2,000 pg/ml

Sensitivity

<10 pg/ml

Function

Cell surface proteoglycan that bears heparan sulfate.

Components

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant human Glypican-5/GPC5

Anticoagulant

heparin or EDTA

Subcellular Location

Cell membrane, GPI-anchor, Extracellular side, Secreted glypican-5, extracellular space

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