Mouse VEGF-B ELISA Kit PicoKine®, Carrier-free

Mouse VEGF-B ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Vegfb in cell culture supernatants, serum and plasma (heparin). . Sensitivity: 2pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Vascular endothelial growth factor B also known as VEGF-B is a protein that, in humans, is encoded by the VEGF-B gene. VEGF-B is a growth factor that belongs to the vascular endothelial growth factor family, of which VEGF-A is the best-known member. In contrast to VEGF-A, VEGF-B plays a less pronounced role in the vascular system: Whereas VEGF-A is important for the formation of blood vessels, such as during development or in pathological conditions, VEGF-B seems to play a role only in the maintenance of newly formed blood vessels during pathological conditions. It also plays an important role on several types of neurons. And it is important for the protection of neurons in the retina and thecerebral cortex during stroke and of motoneurons during motor neuron diseases such as amyotrophic lateral sclerosis.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>70</td><td>68</td><td>79</td><td>79</td><td>74</td><td>5.04</td><td>6.8%</td></tr><tr><td>Sample 2</td><td>247</td><td>261</td><td>242</td><td>260</td><td>252</td><td>8.2</td><td>3.2%</td></tr><tr><td>Sample 3</td><td>978</td><td>875</td><td>882</td><td>968</td><td>925</td><td>47.44</td><td>5.1%</td></tr></table>*number of samples for each test n=16.

Synonyms

Vascular endothelial growth factor B;VEGF-B;VEGF-related factor;VRF;Vegfb;Vrf;

Gene Name

Vascular endothelial growth factor B

Gene ID

22340

UniProt

P49766

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: Q20-A207 & Q20-R148

Tissue Specificity

Abundantly expressed in heart, brain, kidney and skeletal muscle.

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, serum and plasma (heparin).

Detection Range

31.2 pg/ml - 2,000 pg/ml

Sensitivity

<2 pg/ml

Function

Growth factor for endothelial cells. VEGF-B167 binds heparin and neuropilin-1 whereas the binding to neuropilin-1 of VEGF-B186 is regulated by proteolysis. VEGF-B seems to be required for normal heart function in adult but is not required for proper development of the cardiovascular system either during development or for angiogenesis in adults. .

Components

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)