Human ADAMTS4 ELISA Kit PicoKine®, Carrier-free

Human ADAMTS4 ELISA Kit PicoKine® (96 Tests). Quantitate Human ADAMTS4 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 20pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

ADAMTS4, A disintegrin and metalloproteinase with thrombospondin motifs 4 is an enzyme that in humans is encoded by the ADAMTS4 gene. ADAMTS4 is a member of the large ADAMTS family of zinc-dependent proteases. The human ADAMTS4 gene is mapped to chromosome 1 by somatic cell hybrid analysis. The enzyme encoded by this gene lacks a C-terminal TS motif. It is responsible for the degradation of aggrecan, a major proteoglycan of cartilage, and brevican, a brain-specific extracellular matrix protein. The cleavage of aggrecan and brevican suggests key roles of this enzyme in arthritic disease and in the central nervous system, potentially, in the progression of glioma.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>1127</td><td>1186</td><td>1062</td><td>1229</td><td>1151</td><td>62.86</td><td>5.4%</td></tr><tr><td>Sample 2</td><td>4290</td><td>4643</td><td>4014</td><td>4511</td><td>4364</td><td>238.44</td><td>5.4%</td></tr><tr><td>Sample 3</td><td>29063</td><td>29288</td><td>30434</td><td>26599</td><td>28846</td><td>1397.6</td><td>4.8%</td></tr></table>*number of samples for each test n=16.

Synonyms

A disintegrin and metalloproteinase with thrombospondin motifs 4;ADAM-TS 4;ADAM-TS4;ADAMTS-4;3.4.24.82;ADMP-1;Aggrecanase-1;ADAMTS4;KIAA0688;UNQ769/PRO1563;

Gene Name

A disintegrin and metalloproteinase with thrombospondin motifs 4

Gene ID

9507

UniProt

O75173

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: CHO; Immunogen sequence: F213-C685

Tissue Specificity

Expressed in brain, lung and heart. Expressed at very low level in placenta and skeletal muscles. Isoform 2 is detected in osteoarthritic synovium. .

Applications

ELISA

Standard

40 ng/tube

Sample Type

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Detection Range

625 pg/ml - 40,000 pg/ml

Sensitivity

<20 pg/ml

Function

Cleaves aggrecan, a cartilage proteoglycan, and may be involved in its turnover. May play an important role in the destruction of aggrecan in arthritic diseases. Could also be a critical factor in the exacerbation of neurodegeneration in Alzheimer disease. Cleaves aggrecan at the '392-Glu-|-Ala-393' site.

Components

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)