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Mouse CD56 / NCAM-1 / NCAM1 ELISA Kit PicoKine®, Carrier-free

Selected Variant

EK1223-carrier-free

Size: 96 Well With Removable Strips

Mouse CD56 / NCAM-1 / NCAM1 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Ncam1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 20pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Neural Cell Adhesion Molecule, NCAM, also known as the cluster of differentiation CD56, is a hemophilic binding glycoprotein. It is a glycoprotein of Immunoglobulin (Ig) super family. At least 27 alternatively spliced NCAM mRNAs are produced, giving a wide diversity of NCAM isoforms NCAM gene is located at 11q22-q23.This glycoprotein is mainly expressed on the surface of neurons, glia, skeletal muscle and natural killer cells. NCAM has been implicated as having a role in cell–cell adhesion, neurite outgrowth, synaptic plasticity, and learning and memory.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>1344</td><td>1609</td><td>1386</td><td>1481</td><td>1455</td><td>101.82</td><td>6.9%</td></tr><tr><td>Sample 2</td><td>4632</td><td>4852</td><td>4530</td><td>4967</td><td>4745</td><td>173</td><td>3.6%</td></tr><tr><td>Sample 3</td><td>15378</td><td>17338</td><td>16502</td><td>16161</td><td>16344</td><td>703.49</td><td>4.3%</td></tr></table>*number of samples for each test n=16.

Synonyms

Neural cell adhesion molecule 1;N-CAM-1;NCAM-1;CD56;Ncam1;Ncam;

Gene Name

Neural cell adhesion molecule 1

Gene ID

17967

UniProt

P13595

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: L20-T711

Applications

ELISA

Standard

40 ng/tube

Sample Type

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Detection Range

625 pg/ml - 40,000 pg/ml

Sensitivity

<20 pg/ml

Function

This protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc.

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

119427 MW

Specificity

Natural and recombinant mouse CD56

Anticoagulant

heparin or EDTA

Subcellular Location

Isoform 1: Cell membrane; Single-pass type I membrane protein.

Sequence Similarities

Contains 2 fibronectin type-III domains.

Protein Name

Neural cell adhesion molecule 1

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