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Human IL-6R Alpha / IL 6 Receptor ELISA Kit PicoKine®, Carrier-free

Selected Variant

EK0777-carrier-free

Size: 96 Well With Removable Strips

Human IL-6R Alpha / IL 6 Receptor ELISA Kit PicoKine® (96 Tests). Quantitate Human IL6R in cell culture supernatants, serum, plasma (heparin, EDTA, citrate) and urine. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

IL6R alpha (IL6RA) also known as CD126 or IL6R, is a type I cytokine receptor. The IL6 receptor is a protein complex consisting of a IL-6 receptor subunit (IL6R) and interleukin 6 signal transducer Glycoprotein 130. IL6R also denotes the human gene encoding this subunit. Alternatively spliced transcript variants encoding distinct isoforms have been reported. IL6R subunit is also shared by many other cytokines. Interleukin-6 receptor has been shown to interact with Interleukin 6 and Ciliary neurotrophic factor. Ligand binding did not appear to affect IL6R dimerization status. IL6R dimerization occurs both on the cell surface and in solution.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>54</td><td>48</td><td>54</td><td>48</td><td>51</td><td>3</td><td>5.8%</td></tr><tr><td>Sample 2</td><td>271</td><td>254</td><td>262</td><td>234</td><td>255</td><td>13.66</td><td>5.3%</td></tr><tr><td>Sample 3</td><td>821</td><td>928</td><td>965</td><td>877</td><td>897</td><td>54.21</td><td>6%</td></tr></table>*number of samples for each test n=16.

Synonyms

Interleukin 6 receptor ;Interleukin 6 receptor, isoform CRA_a ;IL6R ;hCG_1810911 ;

Gene Name

interleukin 6 receptor

Gene ID

3570

UniProt

A0N0L5

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: L20-D358

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, serum, plasma (heparin, EDTA, citrate) and urine.

Detection Range

31.2 pg/ml - 2,000 pg/ml

Sensitivity

<10 pg/ml

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

51548 MW

Specificity

Natural and recombinant human IL-6R alpha

Anticoagulant

heparin, EDTA or Citrate

Protein Name

Interleukin-6 receptor subunit alpha

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