Human HBEGF ELISA Kit PicoKine®, Carrier-free

Human HBEGF ELISA Kit PicoKine® (96 Tests). Quantitate Human HBEGF in cell culture supernatants, cell lysates, serum, plasma (heparin, EDTA), saliva, urine and human milk. Sensitivity: 2pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

HBEGF (Heparin-binding EGF-like growth factor), also known as HEGFL or DTR, is a member of the EGF family of proteins that in humans is encoded by the HBEGF gene. The HBEGF gene is assigned to chromosome 5, thus confirming the assignment of the gene on the basis of its role in relation to diphtheria toxin susceptibility. HB-EGF is an 87 amino acid glycoprotein which displays highly regulated gene expression. It has been shown to play a role in wound healing, cardiac hypertrophy and heart development and function. HB-EGF binding and activation of EGF receptors plays a critical role during cardiac valve tissue development and the maintenance of normal heart function in adults.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>16</td><td>14</td><td>15</td><td>15</td><td>15</td><td>0.7</td><td>4.6%</td></tr><tr><td>Sample 2</td><td>77</td><td>73</td><td>73</td><td>83</td><td>76</td><td>4.09</td><td>5.3%</td></tr><tr><td>Sample 3</td><td>282</td><td>268</td><td>286</td><td>279</td><td>278</td><td>6.68</td><td>2.4%</td></tr></table>*number of samples for each test n=16.

Synonyms

Proheparin-binding EGF-like growth factor;Heparin-binding EGF-like growth factor;HB-EGF;HBEGF;Diphtheria toxin receptor;DT-R;HBEGF;DTR, DTS, HEGFL;

Gene Name

Proheparin-binding EGF-like growth factor

Gene ID

1839

UniProt

Q99075

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: D63-L148

Applications

ELISA

Standard

10 ng/tube

Sample Type

culture supernatants, cell lysates, serum, plasma (heparin, EDTA), saliva, urine and human milk.

Detection Range

7.8 pg/ml - 500 pg/ml

Sensitivity

<10 pg/ml

Function

Growth factor that mediates its effects via EGFR, ERBB2 and ERBB4. Required for normal cardiac valve formation and normal heart function. Promotes smooth muscle cell proliferation. May be involved in macrophage-mediated cellular proliferation. It is mitogenic for fibroblasts, but not endothelial cells. It is able to bind EGF receptor/EGFR with higher affinity than EGF itself and is a far more potent mitogen for smooth muscle cells than EGF. Also acts as a diphtheria toxin receptor.

Components

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

23067 MW

Specificity

Natural and recombinant human HBEGF

Anticoagulant

heparin or EDTA

Subcellular Location

Heparin-binding EGF-like growth factor: Secreted, extracellular space. Mature HB-EGF is released into the extracellular space and probably binds to a receptor.