Human IL-20/Interleukin-20 ELISA Kit PicoKine®, Carrier-free

Human IL-20 ELISA Kit PicoKine® (96 Tests). Quantitate Human IL20 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Interleukin-20 (IL-20) is a protein belonging to the IL-10 family of cytokines. IL-20 is produced by activated keratinocytes and monocytes and transmits an intracellular signal through two distinct cell-surface receptor complexes on keratinocytes and other epithelial cells. By radiation hybrid analysis, the IL20 gene was mapped to chromosome 1q32, where it is tightly linked to the IL10, IL19, and MDA7 genes within a 195-kb region, the IL10 family cytokine cluster. IL-20 regulates proliferation and differentiation of keratinocytes during inflammation, particularly inflammation associated with the skin. In addition, IL-20 also causes cell expansion of multipotential hematopoietic progenitor cells.

Reproducibility

<table cellpadding="3"><tr><th>Lots</th><th>Lot 1 (pg/ml)</th><th>Lot 2 (pg/ml)</th><th>Lot 3 (pg/ml)</th><th>Lot 4 (pg/ml)</th><th>Mean (pg/ml)</th><th>Standard Deviation</th><th>CV (%)</th></tr><tr><td>Sample 1</td><td>97</td><td>97</td><td>98</td><td>99</td><td>97</td><td>0.82</td><td>0.8%</td></tr><tr><td>Sample 2</td><td>693</td><td>639</td><td>709</td><td>709</td><td>687</td><td>28.75</td><td>4.1%</td></tr><tr><td>Sample 3</td><td>1747</td><td>1963</td><td>1758</td><td>1970</td><td>1859</td><td>107.09</td><td>5.7%</td></tr></table>*number of samples for each test n=16.

Synonyms

Interleukin-20;IL-20;Cytokine Zcyto10;IL20;ZCYTO10;UNQ852/PRO1801;

Gene Name

Interleukin-20

Gene ID

50604

UniProt

Q9NYY1

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: L25-E176

Tissue Specificity

Expressed in most tissues and five major cell types: epithelial cells (primarily skin, buccal mucosa, tongue, nasal mucosa, lung, ureter, breast, prostate, fallopian tube, and adrenal gland), myoepithelial cells (mainly prostate), endothelial cells (mainly in small vessels or capillaries), macrophages, and skeletal muscle. Isoform 2 was detected in the lung tissue only. .

Applications

ELISA

Standard

10 ng/tube

Sample Type

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Detection Range

62.5 pg/ml - 4,000 pg/ml

Sensitivity

<10 pg/ml

Function

Proinflammatory and angiogenic cytokine that may be involved in epidermal function and psoriasis. Angiogenic and proliferative activities are antagonized by IL10. May act through STAT3. .

Components

Storage Conditions

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)