Anti-BNIP3 Rabbit Monoclonal Antibody, Carrier-free

Boster Bio Anti-BNIP3 Rabbit Monoclonal Antibody catalog # M01469. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.

Product Specifications

Synonyms

BCL2/adenovirus E1B 19 kDa protein-interacting protein 3;BNIP3;NIP3;

Gene Name

BCL2/adenovirus E1B 19 kDa protein-interacting protein 3

UniProt

Q12983

Host

Rabbit

Reactivity

Human,Mouse,Rat

Immunogen

A synthesized peptide derived from human BNIP3

Clonality

Monoclonal

Clone

Clone: AAEI-2

Tissue Specificity

Detected in the flagellum and head region of spermatozoa (at protein level). .

Applications

WB,IHC,ICC,IF

Purification

Affinity-chromatography

Concentration

Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Form

Liquid

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Function

Apoptosis-inducing protein that can overcome BCL2 suppression. May play a role in repartitioning calcium between the two major intracellular calcium stores in association with BCL2. Involved in mitochondrial quality control via its interaction with SPATA18/MIEAP: in response to mitochondrial damage, participates to mitochondrial protein catabolic process (also named MALM) leading to the degradation of damaged proteins inside mitochondria. The physical interaction of SPATA18/MIEAP, BNIP3 and BNIP3L/NIX at the mitochondrial outer membrane regulates the opening of a pore in the mitochondrial double membrane in order to mediate the translocation of lysosomal proteins from the cytoplasm to the mitochondrial matrix. Plays an important role in the calprotectin (S100A8/A9)-induced cell death pathway. .

Components

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Storage Conditions

Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

21541 MW

Observed Molecular Weight

314 kDa

Fragment

Rabbit IgG

Applications Notes

WB 1:500-1:2000<br>IHC 1:50-1:200<br>ICC/IF 1:50-1:200

Subcellular Location

Mitochondrion. Mitochondrion outer membrane; Single-pass membrane protein. Coexpression with the EIB 19- kDa protein results in a shift in NIP3 localization pattern to the nuclear envelope. Colocalizes with ACAA2 in the mitochondria. Colocalizes with SPATA18 at the mitochondrion outer membrane.