Rat MIP-3 Alpha/CCL20 ELISA Kit PicoKine®

Macrophage Inflammatory Protein 3alpha (MIP3alpha), also called Chemokine, cc motif, ligand 20 (CCL20) . The MIP-3alpha/CCL20 gene was cloned and sequenced, revealing a four exon, three intron structure, and was localized by FISH analysis to 2q35-q36. MIP3alpha is predominantly expressed in lymph nodes, appendix, PBL, fetal liver, fetal lung and several cell lines. MIP3alpha/CCL20 and its receptor CCR6 are markedly up-regulated in psoriasis, and they may play a role in the recruitment of T cells to lesional psoriatic skin. And Alanine MIP-3alpha and Serine MIP-3alpha, the two forms of MIP3alpha, that differ by one amino acid at the predicted signal peptide cleavage site. Both of them were chemically synthesized and tested for biological activity. And both flu antigen plus IL-2-activated CD4 (+) and CD8 (+) T lymphoblasts and cord blood-derived dendritic cells responded to Ser and Ala MIP-3alpha.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 93 88 98 96 93 3.76 4% Sample 2 283 261 288 294 281 12.45 4.4% Sample 3 782 809 756 768 778 19.74 2.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 93 88 98 96 93 3.76 4% Sample 1 93 88 98 96 93 3.76 4% Sample 2 283 261 288 294 281 12.45 4.4% Sample 2 283 261 288 294 281 12.45 4.4% Sample 3 782 809 756 768 778 19.74 2.5% Sample 3 782 809 756 768 778 19.74 2.5%

C-C motif chemokine 20; Beta-chemokine exodus-1; CC chemokine LARC; CC chemokine ST38; Liver and activation-regulated chemokine; Macrophage inflammatory protein 3 alpha; MIP-3-alpha; Small-inducible cytokine A20; Ccl20; Scya20, St38

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 93 283 782 90 270 852 Standard deviation 4.83 14.15 43.79 5.31 15%6 56.23 CV (%) 5.2% 5% 5.6% 5.9% 5.8% 6.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 93 283 782 90 270 852 Standard deviation 4.83 14.15 43.79 5.31 15%6 56.23 CV (%) 5.2% 5% 5.6% 5.9% 5.8% 6.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 93 283 782 90 270 852 Mean (pg/ml) 93 283 782 90 270 852 Standard deviation 4.83 14.15 43.79 5.31 15%6 56.23 Standard deviation 4.83 14.15 43.79 5.31 15%6 56.23 CV (%) 5.2% 5% 5.6% 5.9% 5.8% 6.6% CV (%) 5.2% 5% 5.6% 5.9% 5.8% 6.6%

Chemotactic factor that attracts lymphocytes and, slightly, neutrophils, but not monocytes (By similarity) . May play a role in modulating inflammatory cell recruitment to the CNS and therefore contribute to tissue injury in ischemic stroke and autoimmune diseases. .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.021 0.075 0.12 0.231 0.43 0.838 1.623 2.853 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.021 0.075 0.12 0.231 0.43 0.838 1.623 2.853 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.021 0.075 0.12 0.231 0.43 0.838 1.623 2.853 O.D. 0.021 0.075 0.12 0.231 0.43 0.838 1.623 2.853

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.