Anti-MAFG Antibody Picoband®, Carrier-free

Boster Bio Anti-MAFG Antibody Picoband® catalog # A04636-2. Tested in Flow Cytometry, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

Transcription factor MafG is a bZip Maf transcription factor protein that in humans is encoded by the MAFG gene. Globin gene expression is regulated through nuclear factor erythroid-2 (NFE2) elements located in enhancer-like locus control regions positioned many kb upstream of alpha- and beta-gene clusters (summarized by Blank et al., 1997 [PubMed 9166829]). NFE2 DNA-binding activity consists of a heterodimer containing a ubiquitous small Maf protein (MafF, MIM 604877; MafG; or MafK, MIM 600197) and the tissue-restricted protein p45 NFE2 (MIM 601490). Both subunits are members of the activator protein-1-like superfamily of basic leucine zipper (bZIP) proteins (see MIM 165160).

Synonyms

Solute carrier family 12 member 5; Electroneutral potassium-chloride cotransporter 2; K-Cl cotransporter 2; hKCC2; Neuronal K-Cl cotransporter; SLC12A5; KCC2; KIAA1176

Gene Name

solute carrier family 12 member 5

Gene ID

4097

UniProt

O15525

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human MAFG, identical to the related mouse and rat sequences.

Clonality

Polyclonal

Tissue Specificity

Brain specific. Detected in neuronal cells.

Applications

Flow Cytometry,WB

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Mediates electroneutral potassium-chloride cotransport in mature neurons and is required for neuronal Cl- homeostasis. As major extruder of intracellular chloride, it establishes the low neuronal Cl- levels required for chloride influx after binding of GABA-A and glycine to their receptors, with subsequent hyperpolarization and neuronal inhibition. Involved in the regulation of dendritic spine formation and maturation.

Components

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

References & Citations

1. Blank, V., Kim, M. J., Andrews, N. C. Human MAFG is a functional partner for p45 NF-E2 in activating globin gene expression. Blood 89: 3925-3935, 1997. 2. Blank, V., Knoll, J. H. M., Andrews, N. C. Molecular characterization and localization of the human MafG gene. Genomics 44: 147-149, 1997. 3. Iwata, T., Kogame, K., Toki, T., Yokoyama, M., Yamamoto, M., Ito, E. Structure and chromosome mapping of the human small maf genes MAFG and MAFK. Cytogenet. Cell Genet. 82: 88-90, 1998.

Storage Conditions

At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.