Anti-ACF1/BAZ1A Antibody Picoband®, iFluor647 Conjugate

Boster Bio Anti-ACF1/BAZ1A Antibody Picoband® catalog # A04090-1. Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

Bromodomain adjacent to zinc finger domain protein 1A is a protein that in humans is encoded by the BAZ1A gene. The BAZ1A gene encodes the accessory subunit of the ATP-dependent chromatin assembly factor (ACF), a member of the ISWI ('imitation switch') family of chromatin remodeling complexes.

Synonyms

Muscarinic acetylcholine receptor M1; CHRM1

Gene Name

cholinergic receptor muscarinic 1

Gene ID

11177

UniProt

Q9NRL2

Host

Rabbit

Reactivity

Human,Mouse,Rat,Monkey

Cross Reactivity

No cross-reactivity with other proteins.

Immunogen

E.coli-derived human ACF1/BAZ1A recombinant protein (Position: E1033-H1536).

Clonality

Polyclonal

Tissue Specificity

Sperm. Mainly localized in the tail and in the postacrosomal region but is also found in the midpiece and basal region in a small percentage of sperm cells. Reduced levels found in the sperms of asthenozoospermia and leukocytospermia patients (at protein level). Spleen, lymph nodes, appendix, and fetal liver. Expressed in lymphocytes, T-cells and B-cells but not in natural killer cells, monocytes or granulocytes.

Applications

WB,ICC,IF,Flow Cytometry,ELISA

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is Pi turnover.

Components

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

References & Citations

1. Blosser, T. R., Yang, J. G., Stone, M. D., Narlikar, G. J., Zhuang, X. Dynamics of nucleosome remodelling by individual ACF complexes. Nature 462: 1022-1027, 2009. 2. Bochar, D. A., Savard, J., Wang, W., Lafleur, D. W., Moore, P., Cote, J., Shiekhattar, R. A family of chromatin remodeling factors related to Williams syndrome transcription factor. Proc. Nat. Acad. Sci. 97: 1038-1043, 2000. 3. Collins, N., Poot, R. A., Kukimoto, I., Garcia-Jimenez, C., Dellaire, G., Varga-Weisz, P. D. An ACF1-ISWI chromatin-remodeling complex is required for DNA replication through heterochromatin. Nature Genet. 32: 627-632, 2002.

Storage Conditions

At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Calculated Molecular Weight

211344 MW

Observed Molecular Weight

203 kDa

Fragment

Rabbit IgG

Applications Notes

Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat, Monkey Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human ELISA, 0.1-0.5 μg/ml, -