Anti-VIP Receptor 1/VIPR1 Antibody Picoband®, APC Conjugate

Boster Bio Anti-VIP Receptor 1/VIPR1 Antibody catalog # PA1756. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

VIPR1 (Vasoactive intestinal polypeptide receptor 1), also known as VIPR,HVR1, is a protein that in humans is encoded by the VIPR1 gene. Distinct subsets of neural, respiratory, gastrointestinal, and immune cells bear specific high-affinity G protein-coupled receptors for VIP, such as VIPR1. The VIPR1 gene is mapped on 3p22.1. The VIPR1 gene was found to span approximately 22 kb and to be comprised of 13 exons (ranging from 42 to 1,400 bp) and 12 introns (ranging from 0.3 to 6.1 kb). One encodes a VIP receptor consisting of 460 amino acids and having 7 putative transmembrane domains, as do other G protein-coupled receptors. Patients with idiopathic achalasia show a significant difference in the distribution of SNPs affecting VIPR1.

Synonyms

Vasoactive intestinal polypeptide receptor 1;VIP-R-1;Pituitary adenylate cyclase-activating polypeptide type II receptor;PACAP type II receptor;PACAP-R-2;PACAP-R2;VPAC1;VIPR1;

Gene Name

Vasoactive intestinal polypeptide receptor 1

UniProt

P32241

Host

Rabbit

Reactivity

Human,Mouse,Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human VIP Receptor 1, identical to the related rat and mouse sequences.

Clonality

Polyclonal

Tissue Specificity

In lung, HT-29 colonic epithelial cells, Raji B-lymphoblasts. Lesser extent in brain, heart, kidney, liver and placenta. Not expressed in CD4+ or CD8+ T-cells. Expressed in the T-cell lines HARRIS, HuT 78, Jurkat and SUP-T1, but not in the T- cell lines Peer, MOLT-4, HSB and YT. .

Applications

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Function

This is a receptor for VIP. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase. The affinity is VIP = PACAP-27 > PACAP-38. .

Components

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

References & Citations

1. Couvineau, A., Rouyer-Fessard, C., Darmoul, D., Maoret, J.-J., Carrero, I., Ogier-Denis, E., Laburthe, M. Human intestinal VIP receptor: cloning and functional expression of two cDNA encoding proteins with different N-terminal domains. Biochem. Biophys. Res. Commun. 200: 769-776, 1994. 2. Sreedharan, S. P., Huang, J.-X., Cheung, M.-C., Goetzl, E. J. Structure, expression, and chromosomal localization of the type I human vasoactive intestinal peptide receptor gene. Proc. Nat. Acad. Sci. 92: 2939-2943, 1995.

Storage Conditions

Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.