Welcome to GenPrice! Check out our latest updates.

Shopping Cart (0)

Your cart is empty

Add some products to get started!

Human CSF3R/G-CSF R ELISA Kit PicoKine®

Human CSF3R/G-CSF R ELISA Kit PicoKine® (96 Tests) . Quantitate Human CSF3R in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

The granulocyte colony-stimulating factor receptor (G-CSF-R), also known as CD114 (Cluster of Differentiation 114), is a protein that in humans is encoded by the CSF3R gene. It is mapped to 1p35-p34.3. G-CSF-R is a cell-surface receptor for the granulocyte colony-stimulating factor (G-CSF) . And the G-CSF-R is a transmembrane receptor that consists of an extracellular ligand-binding portion, a transmembrane domain, and the cytoplasmic portion that is responsible for signal transduction. In addition, GCSF-R ligand-binding is associated with dimerization of the receptor and signal transduction through proteins including Jak, Lyn, STAT, andErk1/2.

Anticoagulant

Heparin or EDTA

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 72 77 84 86 79 5.58 7% Sample 2 222 224 255 245 236 13.97 5.9% Sample 3 823 879 872 734 827 57.86 6.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 72 77 84 86 79 5.58 7% Sample 1 72 77 84 86 79 5.58 7% Sample 2 222 224 255 245 236 13.97 5.9% Sample 2 222 224 255 245 236 13.97 5.9% Sample 3 823 879 872 734 827 57.86 6.9% Sample 3 823 879 872 734 827 57.86 6.9%

Synonyms

Granulocyte colony-stimulating factor receptor; G-CSF receptor; G-CSF-R; CD114; CSF3R; GCSFR

Gene Name

CSF3R

Gene ID

1441

UniProt

Q99062

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: E25-P621

Capture Antibody

Monoclonal antibody from mouse

Detection Antibody

Polyclonal antibody from goat

Tissue Specificity

One or several isoforms have been found in myelogenous leukemia cell line KG-1, leukemia U-937 cell line, in bone marrow cells, placenta, and peripheral blood granulocytes. Isoform GCSFR-2 is found only in leukemia U-937 cells. Isoform GCSFR-3 is highly expressed in placenta.

Applications

ELISA

Field of Research

Cancer, Cytokines, Hematopoietic Progenitors, Immunology, Innate Immunity, Oncoproteins, Oncoproteins/Suppressors, Stem Cells, Surface Molecules

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .

Detection Range

31.2 pg/mL - 2,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 72 222 823 69 240 838 Standard deviation 5.54 9.99 59.25 6.27 18.12 67.87 CV (%) 7.7% 4.5% 7.2% 9.1% 6.3% 8.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 72 222 823 69 240 838 Standard deviation 5.54 9.99 59.25 6.27 18.12 67.87 CV (%) 7.7% 4.5% 7.2% 9.1% 6.3% 8.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 72 222 823 69 240 838 Mean (pg/ml) 72 222 823 69 240 838 Standard deviation 5.54 9.99 59.25 6.27 18.12 67.87 Standard deviation 5.54 9.99 59.25 6.27 18.12 67.87 CV (%) 7.7% 4.5% 7.2% 9.1% 6.3% 8.1% CV (%) 7.7% 4.5% 7.2% 9.1% 6.3% 8.1%

Sensitivity

<10 pg/mL

Function

Receptor for granulocyte colony-stimulating factor (CSF3), essential for granulocytic maturation. Plays a crucial role in the proliferation, differientation and survival of cells along the neutrophilic lineage. In addition it may function in some adhesion or recognition events at the cell surface. .

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Product Datasheet

https://www.bosterbio.com/datasheet?sku=EK0766

Product MSDS

https://www.bosterbio.com/msds?sku=EK0766

Calculated Molecular Weight

92156 MW

Specificity

Natural and recombinant human CSF3R

Gene Name Synonym

Granulocyte colony-stimulating factor receptor

Subcellular Location

Isoform 2: Secreted .

Protein Name

Granulocyte colony-stimulating factor receptor

Sample Data

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.028 0.238 0.332 0.512 0.733 1.185 1.722 2.582 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.028 0.238 0.332 0.512 0.733 1.185 1.722 2.582 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.028 0.238 0.332 0.512 0.733 1.185 1.722 2.582 O.D. 0.028 0.238 0.332 0.512 0.733 1.185 1.722 2.582

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
Curated Selection

Explore Other Products

Discover premium biology products from our extensive collection of 20M+ items

Dihydromunduletone
MBS5753016 Inquire

Dihydromunduletone

Ask
View Details
Rat HK2 (Hexokinase 2) ELISA Kit
ELK11248-01 48 Tests

Rat HK2 (Hexokinase 2) ELISA Kit

Ask
View Details
Rat HK2 (Hexokinase 2) ELISA Kit
ELK11248-02 96 Tests

Rat HK2 (Hexokinase 2) ELISA Kit

Ask
View Details
Rat HK2 (Hexokinase 2) ELISA Kit
ELK11248-03 5x 96 Tests

Rat HK2 (Hexokinase 2) ELISA Kit

Ask
View Details
Cetirizine Glycerol Ester Dihydrochloride (Mixture of Diastereomers)
TRC-C281130-5MG 5 mg

Cetirizine Glycerol Ester Dihydrochloride (Mixture of Diastereomers)

Ask
View Details
Bovine Ankyrin repeat domain-containing protein 36A (ANKRD36) ELISA Kit
MBS7228250-01 48 Well

Bovine Ankyrin repeat domain-containing protein 36A (ANKRD36) ELISA Kit

Ask
View Details