Anti-PRDM15 Antibody Picoband®, APC Conjugate

Boster Bio Anti-PRDM15 Antibody Picoband® catalog # A13548-1. Tested in ELISA, Flow Cytometry, IF, IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Specifications

Background

PR/SET domain 15 is a protein that in humans is encoded by the PRDM15 gene. Predicted to enable DNA-binding transcription activator activity, RNA polymerase II-specific; RNA polymerase II cis-regulatory region sequence-specific DNA binding activity; and promoter-specific chromatin binding activity. Predicted to be involved in positive regulation of transcription by RNA polymerase II; regulation of signal transduction; and regulation of stem cell division. Located in nuclear body.

Synonyms

RNA-binding protein 47;RNA-binding motif protein 47;RBM47;

Gene Name

RNA-binding protein 47

Gene ID

63977

UniProt

P57071

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E.coli-derived human PRDM15 recombinant protein (Position: D24-Q1494).

Clonality

Polyclonal

Tissue Specificity

Abundantly expressed in tonsil, lymph node, and trachea; strong expression in prostate; lower expression in thyroid, stomach, and colon. .

Applications

ELISA,Flow Cytometry,IF,IHC,WB

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Form

Lyophilized

Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Function

Can bind to the surface of B-lymphoma cells, but not T- lymphoma cells, consistent with a function as a secreted mediator acting upon B-cells.

Components

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

References & Citations

1. Mzoughi, S., Zhang, J., Hequet, D., Teo, S. X., Fang, H., Xing, Q. R., Bezzi, M., Seah, M. K. Y., Ong, S. L. M., Shin, E. M., Wollmann, H., Wong, E. S. M., Al-Haddawi, M., Stewart, C. L., Tergaonkar, V., Loh, Y.-H., Dunn, N. R., Messerschmidt, D. M., Guccione, E. PRDM15 safeguards naive pluripotency by transcriptionally regulating WNT and MAP-ERK signaling. Nature Genet. 49: 1354-1363, 2017. 2. Shibuya, K., Kudoh, J., Okui, M., Shimizu, N. Identification of a novel zinc finger protein gene (ZNF298) in the GAP2 of human chromosome 21q. Biochem. Biophys. Res. Commun. 332: 557-568, 2005.

Storage Conditions

At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Calculated Molecular Weight

64099 MW

Observed Molecular Weight

169 kDa

Fragment

Rabbit IgG

Applications Notes

Western blot, 0.25-0.5 μg/ml, Human Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human Immunofluorescence, 5 μg/ml, Human Flow Cytometry (Fixed), 1-3 μg/1x1x106 cells, Human ELISA, 0.1-0.5 μg/ml, -