Recombinant Murine Ciliary Neurotrophic Factor
Recombinant Murine Ciliary Neurotrophic Factor
Product Specifications
UniProt
P51642
Source
Escherichia coli
Endotoxin
Less than 1 EU/ug of rMuCNTF as determined by LAL method.
Purity
> 95 % by SDS-PAGE and HPLC analyses.
Bioactivity
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 30 ng/ml, corresponding to a specific activity of > 3.3 x104 IU/mg.
Form
Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 150 mM NaCl, with 5 % Trehalose, 0.02 % Tween-20.
Molecular Weight
Approximately 22.5 kDa, a single non-glycosylated polypeptide chain containing 197 amino acids.
Storage Conditions
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Notes
For research use only.
AA Sequence
AFAEQSPLTL HRRDLCSRSI WLARKIRSDL TALMESYVKH QGLNKNISLD SVDGVPVAST DRWSEMTEAE RLQENLQAYR TFQGMLTKLL EDQRVHFTPT EGDFHQAIHT LTLQVSAFAY QLEELMALLE QKVPEKEADG MPVTIGDGGL FEKKLWGLKV LQELSQWTVR SIHDLRVISS HHMGISAHES HYGAKQM
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