Recombinant Rat Insulinlike growth factor I (rRtIGF-1)
Insulinlike growth factor I, also known as somatomedin C, is the dominant effector of growth hormone and is structurally homologous to proinsulin. It shares 61% aa sequence identity with mature rat IGF-II. Circulating IGF-I is produced by hepatocytes, while local IGF-I is produced by many other tissues in which it has paracrine effects. IGF-I induces the proliferation, migration, and differentiation of a wide variety of cell types during development and postnatally.
Product Specifications
Source
Escherichia coli.
Field of Research
Stem Cell & Developmental Biology
Endotoxin
Less than 1EU/μg of rRtIGF-1 as determined by LAL method.
Purity
>97% by SDS-PAGE and HPLC analyses.
Bioactivity
Measured in a serum free cell proliferation assay using MCF7 human breast adenocarcinoma cells. The ED50 for this effect is typically 0.6-3 ng/mL.
Form
Lyophilized from a 0.2mm filtered concentrated solution in PBS, pH 7.4.
Molecular Weight
Approximately 7.7 kDa, a single non-glycosylated polypeptide chain containing 70 amino acid residues.
Storage Conditions
Notes
For research use only.
Applications Notes
Preservative
Lyophilized from a 0.2mm filtered concentrated solution in PBS, pH 7.4.
AA Sequence
GPETLCGAEL VDALQFVCGP RGFYFNKPTG YGSSIRRAPQ TGIVDECCFR SCDLRRLEMY CAPLKPTKSA
Available Sizes
Frequently Asked Questions
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