Recombinant SARS-CoV-2 Nucleocapsid Protein
Product Specifications
Background
Coronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is a most abundant protein of coronavirus. N protein packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication. Because of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.
Synonyms
SARS-CoV-2 coronavirus NP Protein; SARS-CoV-2 novel coronavirus Nucleoprotein Protein; SARS-CoV-2 np Protein
UniProt
P0DTC2
Accession Number
QHD43423.2
Expression System
HEK293 Cells
Tag
C-His
Sequence
Met1-Ala419
Applications
ELISA
Endotoxin
Please contact us for more information.
Purity
> 90 % as determined by reducing SDS-PAGE.
Bioactivity
Immobilized 2019-nCoV NP Antibody (6G9) -Fc at 5μg/ml (100 μl/well) can bind Recombinant 2019-nCoV Nucleocapsid Protein (Mammalian) -His. The ED50 of 2019-nCoV Nucleocapsid Protein-His is 0.172 ug/ml.
Reconstitution
Not Applicable
Shipping Conditions
This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
Storage Conditions
Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Calculated Molecular Weight
46.4 kDa
Observed Molecular Weight
50 kDa
Formulation
Supplied as a 0.2 μm filtered solution of 50mM Tris-HCl, 150mM NaCl, 50mM Arginine, pH7.5
Species
SARS-CoV-2
Available Sizes
Documents
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