Recombinant Mouse IL-1A/IL-1α Protein

Product Specifications

Background

Mouse Interleukin-1 (IL-1) designates two proteins, IL-1α and IL-1β, which are the products of distinct genes, but recognize the same cell surface receptors. IL-1α and IL-1β are structurally related polypeptides that show approximately 25% homology at the amino acid level. Both proteins are produced by a wide variety of cells in response to stimuli such as those produced by inflammatory agents, infections, or microbial endotoxins. The proteins are synthesized as 31 kDa precursors that are subsequently cleaved into proteins with molecular weights of approximately 17.5 kDa.

Abbreviation

IL-1A; IL-1α

Synonyms

Hematopoietin; IL1F; Hematopoietin-1; IL1; IL1-A; IL1F1; IL-1α; Preinterleukin 1 Alpha; Pro-Interleukin-1-Alpha; Il-1a; Interleukin-1 alpha; IL-1 alpha; Il1a

UniProt

P01582

Accession Number

P01582

Expression System

E.coli

Tag

C-His

Sequence

Ser115-Ser270

Applications

Cell culture

Endotoxin

< 0.1 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

50 2 x 108 IU/mg.8

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.