Recombinant Human CD3 epsilon/CD3E (C-mFC)
Product Specifications
Background
T-Cell Surface Glycoprotein CD3 ε Chain (CD3ε) is a single-pass type I membrane protein. CD3ε contains 1 Ig-like (immunoglobulin-like) domain and 1 ITAM domain. CD3ε is a polypeptide encoded by the CD3E gene on chromosome 11 in humans. The T cell receptor-CD3 complex (TCR/CD3 complex) is involved in T-cell development and several intracellular signal-transduction pathways. This complex is critical for T-cell development and function, and represents one of the most complex transmembrane receptors. The T cell receptor-CD3 complex is unique in having ten cytoplasmic immunoreceptor tyrosine-based activation motifs (ITAMs) . TCR/CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways.
Abbreviation
CD3 epsilon; CD3E
Synonyms
CD3E; CD3e; T-Cell Surface Antigen T3/Leu-4 Epsilon Chain; T-Cell Surface Glycoprotein CD3 Epsilon Chain; T3E
UniProt
P07766
Accession Number
P07766
Expression System
HEK293 Cells
Tag
C-mFC
Sequence
Gln22-Thr48
Applications
ELISA
Field of Research
Immunology; Stem cells
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method.
Purity
> 95 % as determined by reducing SDS-PAGE.
Bioactivity
Immobilized Anti-Human/Monkey CD3E mAb at 2μg/ml (100 μl/well) can bind Human CD3E-mFc.The ED50 of Human CD3E-mFc is 28.99 ng/ml.
Reconstitution
Please refer to the printed manual for detailed information.
Shipping Conditions
This product is provided as lyophilized powder which is shipped with ice packs.
Storage Conditions
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Product Datasheet
https://789.bio/ea/9psc00
Product MSDS
https://789.bio/eb/iPWXn9
Calculated Molecular Weight
28.5 kDa
Observed Molecular Weight
32-40 kDa
Species
Human
Available Sizes
Curated Selection
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