Recombinant Human PCBD1 Protein (His Tag)
Product Specifications
Background
Pterin-4-α-Carbinolamine Dehydratase (PCBD1) is the founding member of the Pterin-4-α-Carbinolamine Dehydratase Family. PCBD1 is involved in Tetrahydrobiopterin biosynthesis. It seems to prevent the formation of 7-Pterins and accelerate the formation of Quinonoid-BH2. Furthermore, PCBD1 regulates the homodimerization of the transcription factor Hepatocyte Nuclear Factor 1 (HNF1) and enhances its transcriptional activity. Defects in PCBD1 are the cause of BH4-Deficient Hyperphenylalaninemia Type D (HPABH4D) . HPABH4D is characterized by the excretion of 7-substituted Pterins in the urine of affected patients.
Abbreviation
PCBD1
Synonyms
4-Alpha-Hydroxy-Tetrahydropterin Dehydratase; DCoH; Dimerization Cofactor of HNF1; Dimerization Cofactor of Hepatocyte Nuclear Factor 1-Alpha; PHS; Phenylalanine Hydroxylase-Stimulating Protein; Pterin Carbinolamine Dehy; Pterin-4-Alpha-Carbinolamine Dehydratase
UniProt
P61457
Accession Number
P61457
Expression System
E.coli
Tag
N-His
Sequence
Ala2-Thr104
Field of Research
Signal Transduction; epigenetics and nuclear signal; metabolism
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method.
Purity
> 95 % as determined by reducing SDS-PAGE.
Bioactivity
Not validated for activity
Reconstitution
Not Applicable
Shipping Conditions
This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
Storage Conditions
Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Calculated Molecular Weight
14.2 kDa
Observed Molecular Weight
14 kDa
Formulation
Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 150mM NaCl, 1mM DTT, pH 8.0.
Species
Human
Available Sizes
Documents
Frequently Asked Questions
More Discoveries
Explore Other Products
Browse additional items from our catalog
