Recombinant Human LYPLA1 Protein (His Tag)
Product Specifications
Background
Acyl-Protein Thioesterase 1 (APT-1) is lysophospholipase that belongs to the AB hydrolase 2 family. Lysophospholipases are enzymes that act on biological membranes to regulate the multifunctional lysophospholipids. APT-1 performs on biological membranes to regulate the multifunctional lysophospholipids. It hydrolyzes lysophosphatidylcholine in both monomeric and micellar forms. It hydrolyzes fatty acids from S-acylated cysteine residues in proteins such as trimeric G alpha proteins or HRAS; in addition, it also has depalmitoylating activity and low lysophospholipase activity.
Abbreviation
LYPLA1
Synonyms
APT-1; APT1; Acyl-Protein Thioesterase 1; LPL-I; LPL1; LYPLA1; LysoPLA I; Lysophospholipase 1; Lysophospholipase I; hAPT1
UniProt
O75608
Accession Number
O75608
Expression System
E.coli
Tag
N-His
Sequence
Met 1-Asp230
Field of Research
Signal Transduction; Cancer; metabolism
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method.
Purity
> 95 % as determined by reducing SDS-PAGE.
Bioactivity
Not validated for activity
Reconstitution
Not Applicable
Shipping Conditions
This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
Storage Conditions
Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Calculated Molecular Weight
26.8 kDa
Observed Molecular Weight
25 kDa
Formulation
Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 100mM NaCl, 1mM DTT, 10% Glycerol, pH 8.0.
Species
Human
Available Sizes
Documents
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