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Recombinant Human MTHFS Protein (His Tag)

Product Specifications

Background

5-formyltetrahydrofolate cyclo-ligase (MTHFS) belongs to the 5-formyltetrahydrofolate cyclo-ligase family. It is an enzyme that catalyzes the conversion of 5-formyltetrahydrofolate to 5,10-methenyltetrahydrofolate, contributes to tetrahydrofolate metabolism. MTHFS helps regulate carbon flow through the folate-dependent one-carbon metabolic network that supplies carbon for the biosynthesis of purines, thymidine and amino acids. An increased activity of the encoded protein can result in an increased folate turnover rate and folate depletion.

Abbreviation

MTHFS

Synonyms

5,10-methenyl-tetrahydrofolate synthetase;5-formyltetrahydrofolate cyclo-ligase; MTHFS; Methenyl-THF synthetase

UniProt

P49914

Accession Number

P49914

Expression System

E.coli

Tag

C-His

Sequence

Met 1-Ala203

Field of Research

Signal Transduction; Cancer; metabolism

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

24.3 kDa

Observed Molecular Weight

28 kDa

Formulation

Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 200mM Nacl, 1mM DTT, 50% Glycerol, pH 8.0.

Species

Human

Available Sizes

Frequently Asked Questions

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