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His-Tag Monoclonal Antibody

Product Specifications

Background

The 6xHis tag, also known as polyhistidine tag, His6 tag and/or hexa histidine tag, is an amino acid motif consisting of at least 6 histidine residues fused to the carboxyl (C-) or amino (N-) terminus of a target protein in transfected cells. It is one of the simplest and most widely used purification tags in the world.

Abbreviation

His-Tag

Host

Mouse

Reactivity

All

Immunogen

Synthetic peptide corresponding to 6His tag conjugated to keyhole limpet haemocyanin.

Target

6 His epitope tag; Hexa His tag; HHHHHH epitope tag; HHHHHH tag; His tag; Polyhistidine Tag

Clonality

Monoclonal

Clone

1A5

Conjugation

Unconjugated

Applications

WB; IP; FCM

Purification

Antigen Affinity Purification

Concentration

1 mg/mL

Dilution

WB 1:1000-2000; IP 3ug/sample; FCM 0.2ug

Buffer

PBS with 0.02% sodium azide,1% protective protein and 50% glycerol, pH7.4

Shipping Conditions

The product is shipped with ice pack, upon receipt, store it immediately at the temperature recommended.

Storage Conditions

Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

Isotype

IgG1

Available Sizes

Curated Selection

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