Phospho-IkB alpha (Ser32/S36) Polyclonal Antibody

Product Specifications

Background

Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription.

Abbreviation

IκB-α (phospho Ser32/36)

UniProt

P25963

Host

Rabbit

Reactivity

Human; Mouse; Rat; Monkey

Immunogen

Synthesized peptide derived from human IκB-α around the phosphorylation site of Ser32/36

Target

I kappa B alpha; I-kappa-B-alpha; IkappaBalpha; IkB-alpha; IKBA; IKBA; IKBalpha; MAD 3; MAD3; Major histocompatibility complex enhancer-binding protein MAD3; NF kappa B inhibitor alpha; NF-kappa-B inhibitor alpha; NFKBI; NFKBIA; Nuclear factor of kappa light chain gene enhancer in B cells; Nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor alpha

Clonality

Polyclonal

Conjugation

Unconjugated

Applications

WB; IHC-p; IF

Field of Research

Cancer; Epigenetics and Nuclear Signaling; Immunology; Signal Transduction

Purification

Affinity purification

Concentration

1 mg/mL

Dilution

WB 1:500-1:2000; IHC 1:100-1:300; IF 1:200-1:1000

Buffer

PBS with 0.02% sodium azide, 0.5% protective protein and 50% glycerol, pH7.4

Shipping Conditions

The product is shipped with ice pack, upon receipt, store it immediately at the temperature recommended.

Storage Conditions

Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

Calculated Molecular Weight

36kDa

Observed Molecular Weight

34kDa

Citation 01

https://www.sciencedirect.com/science/article/pii/S0753332223000823

Citation 02

https://pubs.acs.org/doi/abs/10.1021/acs.jafc.2c04970

Citation 03

https://www.sciencedirect.com/science/article/pii/S0014299921005859

Citation 04

https://onlinelibrary.wiley.com/doi/abs/10.1111/odi.14218

Citation 05

https://journals.sagepub.com/doi/abs/10.1177/0960327121993224

Citation 06

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9320209/

Citation 07

https://onlinelibrary.wiley.com/doi/abs/10.1111/jfbc.13876

Isotype

IgG

Available Sizes

Curated Selection

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