Total Glutathione (T-GSH) /Oxidized Glutathione (GSSG) Colorimetric Assay Kit
Product Specifications
Target
T-GSH; GSSG
Type
Colorimetric method
Field of Research
Oxidative stress
Assay Principle
GSSG is reduced to GSH by glutathione reductase, and GSH can react with DTNB to produce GSSG and yellow TNB. The amount of total glutathione (GSSG+GSH) determines the amount of yellow TNB. Thus the total glutathione can be calculated by measuring the OD value at 412 nm. The content of GSSG can be determined by first removing GSH from the sample with appropriate reagent and then using the above reaction principle.
Assay Performance Time
180 min
Sample Type
Serum; plasma; animal tissue; red blood cells; cultured cells
Detection Range
0.12-30 μmol/L T-GSH
Sensitivity
0.12 μmol/L T-GSH
Shipping Conditions
Ice bag
Storage Conditions
This product can be stored at 2~8°C/-20°C for 12 months with shading light. Please refer to the manual for the specific storage condition of the components.
Citation 01
https://www.sciencedirect.com/science/article/pii/S0308814621004301
Citation 02
https://pubs.rsc.org/en/content/articlelanding/2022/fo/d1fo03577k
Citation 03
https://sfamjournals.onlinelibrary.wiley.com/doi/abs/10.1111/1462-2920.15397
Citation 04
https://www.tandfonline.com/doi/abs/10.1080/13880209.2022.2070221
Citation 05
https://porcinehealthmanagement.biomedcentral.com/articles/10.1186/s40813-022-00275-w
Citation 06
https://onlinelibrary.wiley.com/doi/abs/10.1002/jsfa.11611
Citation 07
https://www.tandfonline.com/doi/abs/10.1080/21655979.2021.2024319
Inter CV
4.7%
Intra CV
0.9%
Detection Instrument
Spectrophotometer
Available Sizes
Documents
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