Human HMGB-1 (High Mobility Group Protein B1) CLIA Kit
Product Specifications
Abbreviation
HMGB-1
UniProt
P09429
Cross Reactivity
This kit recognizes Human HMGB-1 in samples.No significant cross-reactivity or interference between Human HMGB-1 and analogues was observed
Target
HMGB1; HMG1; HMG3; SBP-1; Amphoterin
Type
Sandwich-ELISA
Applications
CLIA
Field of Research
Cancer; Epigenetics and Nuclear Signaling
Assay Principle
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human HMGB-1. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human HMGB-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human HMGB-1, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human HMGB-1. You can calculate the concentration of Human HMGB-1 in the samples by comparing the RLU value of the samples to the standard curve.
Assay Performance Time
3 h 30 min
Sample Type
Serum, plasma and other biological fluids
Detection Range
12.5-800 pg/mL
Precision
Both intra-CV and inter-CV are < 10%.
Recovery
80%-120%
Sensitivity
7.5 pg/mL
Shipping Conditions
Ice bag
Storage Conditions
2-8°C/-20°C,6 months
Species
Human
Recommended Usage
This ELISA kit applies to the in vitro quantitative determination of Human HMGB-1 concentrations in serum, plasma and other biological fluids.
Available Sizes
Frequently Asked Questions
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