Human NOS2/iNOS (Nitric Oxide Synthase 2, Inducible) CLIA Kit
Product Specifications
Abbreviation
NOS2; iNOS
UniProt
P35228
Cross Reactivity
This kit recognizes Human NOS2/iNOS in samples.No significant cross-reactivity or interference between Human NOS2/iNOS and analogues was observed
Target
NOS2A; HEP-NOS; I-NOS
Type
Sandwich-ELISA
Applications
CLIA
Field of Research
Cancer; Metabolism; Neuroscience
Assay Principle
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human NOS2/iNOS. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human NOS2/iNOS and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human NOS2/iNOS, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human NOS2/iNOS. You can calculate the concentration of Human NOS2/iNOS in the samples by comparing the RLU value of the samples to the standard curve.
Assay Performance Time
3 h 30 min
Sample Type
Serum, plasma and other biological fluids
Detection Range
31.25-2000 pg/mL
Precision
Both intra-CV and inter-CV are < 10%.
Recovery
80%-120%
Sensitivity
18.75 pg/mL
Shipping Conditions
Ice bag
Storage Conditions
2-8°C/-20°C,6 months
Product Datasheet
https://789.bio/ea/wx5c88
Product MSDS
https://789.bio/eb/a94teL
Species
Human
Recommended Usage
This ELISA kit applies to the in vitro quantitative determination of Human NOS2/iNOS concentrations in Serum, plasma and other biological fluids.
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