Vimentin (phospho-S56) polyclonal antibody

Product Specifications

Background

Phosphorylation of Vimentin induces disassembly of Vimentin intermediate filaments in vivo and in vitro. Binding of 14-3-3 depends on Vimentin phosphorylation and requires the phosphopeptide binding domain of 14-3-3, which is an amino terminal head domain consisting of amino acids 1-96. Phosphorylated Vimentin sequesters 14-3-3 and limits its availability to other target proteins, which can affect intracellular signaling processes that require 14-3-3. The amino-terminal domain of Vimentin is the target site for several protein kinases, including Rho kinase and PKC. Ser 38 and Ser 71 of Vimentin are the major sites of phosphorylation by Rho kinase. The disruption of subcellular compartmentalization of interphase cells leads to PKC-mediated phosphorylation of Vimentin. Thus, targeting of activated PKC, coupled with the reorganization of intracellular membranes, which contain phospholipids essential for activation, leads to the mitosis-specific phosphorylation of Vimentin.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Vimentin; VIM

Swiss Prot

P08670

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic phosphopeptide derived from human Vimentint around the phosphorylation site of Serine 56.

Conjugation

Unconjugated

Related Pathways

Neuronal and Glial Cell Marker Atlas, Contribution of ECM and Cytoskeletal Factors to EMT

Applications

Dilution

WB: 1:500~1:1000

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Phosphorylated

Molecular Weight

~ 57 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

P-Vimentin (S56) polyclonal antibody detects endogenous levels of Vimentin protein when phosphorylated at Ser56.

Applications Notes

Western blot (WB) analysis of p-Vimentin (S56) pAb at 1:500 dilution Lane1:MCF-7 whole cell lysate (40µg) Lane2:A375 whole cell lysate (40µg) Lane3:C6 whole cell lysate (40µg) Lane4:The testis tissue lysate of Mouse (40µg) Lane5:Hela whole cell lysate (40µg)