Human β-EP (Beta-Endorphin) ELISA Kit

Product Specifications

Abbreviation

Β-EP

UniProt

P01189

Cross Reactivity

This kit recognizes Human β-EP in samples.No significant cross-reactivity or interference between Human β-EP and analogues was observed

Target

B-EP

Type

Competitive-ELISA

Applications

ELISA

Field of Research

Cancer; Metabolism; Neuroscience; Signal transduction

Assay Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Human β-EP. During the reaction, Human β-EP in the sample or standard competes with a fixed amount of Human β-EP on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Human β-EP. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Human β-EP in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Assay Performance Time

2 h 30 min

Sample Type

Serum, plasma and other biological fluids

Detection Range

15.63-1000 pg/mL

Precision

Both intra-CV and inter-CV are < 10%.

Recovery

80%-120%

Sensitivity

9.38 pg/mL

Shipping Conditions

Ice bag

Storage Conditions

2-8°C,12 months

Citation 01

https://www.sciencedirect.com/science/article/pii/S1382668922001727

Citation 02

https://www.nature.com/articles/s41398-018-0263-y

Citation 03

https://www.nature.com/articles/s41401-018-0202-8

Citation 04

https://www.nature.com/articles/s41401-019-0241-9

Citation 05

https://www.sciencedirect.com/science/article/pii/S0143417922000208

Citation 06

https://www.hindawi.com/journals/mi/aa/965925/abs/

Citation 07

https://www.sciencedirect.com/science/article/pii/S0091305718303691

Citation 08

https://journals.viamedica.pl/endokrynologia_polska/article/view/71523

Detection Instrument

Microplate reader

Species

Human

Recommended Usage

This ELISA kit applies to the in vitro quantitative determination of Human β-EP concentrations in serum, plasma and other biological fluids.

Available Sizes

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