Anti-Angiotensin Converting Enzyme Antibody

Mouse Monoclonal Antibody specific to Angiotensin Converting Enzyme Human

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

ACE, EC 3.2.1.-, EC 3.4.15.1, Dipeptidyl carboxypeptidase I, Kininase II, CD antigen CD143 [Cleaved into: Angiotensin-converting enzyme, soluble form]

Gene Name

ACE

NCBI Gene ID

UniProt

P12821

Cellular Locus

[Angiotensin-converting enzyme, soluble form]: Secreted.; Cell membrane; Single-pass type I membrane protein. Cytoplasm

Host

Mouse

Reactivity

Human, Mouse, Bovine

Immunogen

ACE purified from rat lung.

Target Antigen

Angiotensin-converting enzyme

Target

Angiotensin Converting Enzyme

Clonality

Monoclonal

Isotype

IgM

Type

Antibody

Applications

ELISA, IHC, FACS

Field of Research

Enzymes

Purification Method

PEG Purified

Concentration

Lot Specific

Dilution

Dilute in PBS or medium that is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

Converts angiotensin I to angiotensin II by release of the terminal His-Leu, this results in an increase of the vasoconstrictor activity of angiotensin. Also able to inactivate bradykinin, a potent vasodilator. Has also a glycosidase activity which releases GPI-anchored proteins from the membrane by cleaving the mannose linkage in the GPI moiety.

Additionnal Information

This antibody may be used in ELISA, <br><br>Immunohistochemistry, and flow cytometry for detection of ACE or ACE-positive cells. Other applications are under investigation.

Storage Conditions

These antibodies are stable for at least one (1) year at -20°C to -70°C. Store product in appropriate aliquots to avoid multiple freeze-thaw cycles.

Specificity

This antibody reacts specifically withÂ human, bovine, and murine ACE andÂ with ACE-positive endothelial cells.<h3>DILUTION INSTRUCTIONS</h3>Dilute in PBS or medium which isÂ identical to that used in the assayÂ system.<h3>STORAGE AND STABILITY</h3>These antibodies are stable for at leastÂ one (1) year at -20oC to -70oC. StoreÂ product in appropriate aliquots to avoidÂ multiple freeze-thaw cycles.<h3>REFERENCE</h3>Auerbach, R. et al. (1982) Proc NatlAcad Sci USA 79: 7891.For in vitro investigational use only. NotÂ for use in therapeutic or diagnosticÂ procedures<h3>