Human Noggin Protein
Recombinant Human Noggin protein
Product Specifications
Product Name Alternative
SYM1, SYNS1, NOG.
Source
Sf9, Baculovirus cells
Purity
Greater than 95.0% as determined by: (a) Analysis by RP-HPLC (b) Analysis by SDS-PAGE.
Bioactivity
Measured by its ability to inhibit BMP-4-induced alkaline phosphatase production by ATDC5 mouse chondrogenic cellsans was fount to be 0.04‐0.2 μg/mL in the presence of 50 ng/mL of Recombinant Human BMP‐4.
Form
Sterile Filtered White lyophilized (freeze-dried) powder.
Solubility
It is recommended to reconstitute the lyophilized Noggin in sterile 18MΩ-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions.
Storage Conditions
Stability: Lyophilized Noggin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Noggin should be stored at 4°C between 2-7 days and for future use below -18°C.Please prevent freeze-thaw cycles
Notes
For research use only.
Applications Notes
Cytokines And Growth Factors
Preservative
Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH 7.4 and 0.02 % Tween-20 and 5% trehalose.
AA Sequence
QHYLHIRPAP SDNLPLVDLI EHPDPIFDPK EKDLNETLLR SLLGGHYDPG FMATSPPEDR PGGGGGAAGG AEDLAELDQL LRQRPSGAMP SEIKGLEFSE GLAQGKKQRL SKKLRRKLQM WLWSQTFCPV LYAWNDLGSR FWPRYVKVGS CFSKRSCSVP EGMVCKPSKS VHLTVLRWRC QRRGGQRCGW IPIQYPIISE CKCSC
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