Cuff Peptide
Cuff Peptide
Product Specifications
Product Name Alternative
CG13190, Dmel\CG13190, Protein cutoff
UniProt
Q9V629
Form
Lyophilized
Storage Conditions
Store at -20°C. When reconstituted minimize freezing and thawing.
Notes
For research use only.
Applications Notes
Cuff Antibody ships at 0.5 mg/mL and the cuff Peptide ships as a 100 μg pellet. When peptide is reconstituted in 200 μL water, the concentration will be 0.5 mg/mL, identical to that of the antibody. The best starting ratio would be 1:1 (which means molar excess of peptide relative to antibody when identical volumes are mixed) . Mix equal volume of peptide and antibody at the required dilution and leave at ambient temperature for 20 minutes. Then incubate and develop in parallel two blots: one with the antibody and one with the antibody which has been pre-absorbed with the provided peptide.
Tested Applications
Blocking
NCBI Accession Number
NP_610709.1
Preservative
Cuff Peptide is supplied as 100 μg of dried peptide. When the peptide is reconstituted in 200 μL water, the concentration will be 0.5 mg/mL.
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