Anti-Rad50 Antibody

Mouse Monoclonal Antibody specific to Rad50

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

hRAD50, EC 3.6.-.-

Gene Name

RAD50

NCBI Gene ID

UniProt

Q92878

Cellular Locus

Nucleus, Chromosome, telomere, Chromosome

Host

Mouse

Reactivity

Human

Immunogen

GST fusion protein corresponding to the complete coding region (aa 1-425) of Rad50 expressed in E. coli.

Target Antigen

DNA repair protein RAD50

Target

Rad50

Clonality

Monoclonal

Isotype

IgG1

Type

Antibody

Applications

WB, ICC/IF, IHC-P, IP, IHC, in vitro

Field of Research

Cancer research

Purification Method

Purified by Protein G affinity chromatography

Concentration

Lot Specific

Dilution

Dilute in PBS or medium which is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

Component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA rPubMed:10888888, PubMed:11741547, PubMed:15064416, PubMed:9590181, PubMed:9651580, PubMed:9705271}.

Additionnal Information

Immunoblotting, <br><br>Immunoprecipitation: use at 0.1-2 ug/mL. <br><br>Positive control: Raji cells.

Storage Conditions

This antibody is stable for at least one (1) year at -70°C. Avoid multiple freeze- thaw cycles.

Specificity

This antibody recognizes human Rad50, a 153 kD protein involved in DNA double strand break repair. Rad50 is associated with Mre11 and p95 (Nibrin) to form a multiprotein complex. Following DNA damage, Rad50 and Mre11 redistribute and are co-localized within the nucleus of damaged cells.