NET1/ARHGEF8 (Internal) Antibody
Goat polyclonal antibody to NET1
Product Specifications
Product Name Alternative
Anti NET1 antibody, anti ARHGEF8 antibody, anti neuroepithelial cell transforming gene 1 antibody, anti NET1A antibody, anti Rho guanine nucleotide exchange factor (GEF) 8 antibody, anti guanine nucleotide regulatory protein (oncogene) antibody, anti neuroepithelioma transforming gene 1 antibody, anti Net1 proto-oncogene protein antibody, anti small GTP-binding protein regulator antibody
Reactivity
Human
Target
NET1 / ARHGEF8 (Internal)
Clonality
Polyclonal
Conjugation
Unconjugated
Sequence
DKLEYLDEKQRDPR
Field of Research
Epigenetics, Neuroscience
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Dilution
ELISA: 1:16000, WB: 0.3 μg/ml, IHC-P: 2.5μg/ml
Molecular Weight
67.7; 61.9
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.IHC: In paraffin embedded Human Breast shows cytoplasm staining of myoepithelial cells of ducts. Recommended concentration, 2.5ug/ml.WB: Preliminary experiments gave an approx 80-90kDa double band in Human Placenta lysates after 0.3 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 67.7kDa according to NP_001040625.1. The 80-90kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
Prediction Reactivity
Canine, Mouse, Rat
Tested Applications
ELISA, IHC
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Entrez
10276
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