Rat Permeability Glycoprotein (Pgp) ELISA Kit

CD243 ELISA Kit, ABCB1 ELISA Kit, ABC20 ELISA Kit, CLCS ELISA Kit, GP170 ELISA Kit, MDR1 ELISA Kit, PGY1 ELISA Kit, P-Glycoprotein ELISA Kit, Multidrug resistance protein 1 ELISA Kit, ATP-Binding Cassette, Sub-Family B (MDR/TAP), Member 1 ELISA Kit, Colchicin Sensitivity ELISA Kit

Standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat Pgp. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat Pgp. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat Pgp, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat Pgp in the samples is then determined by comparing the OD of the samples to the standard curve