Rat C-Ros Oncogene 1, Receptor Tyrosine Kinase (ROS1) ELISA Kit

MCF3 ELISA Kit, ROS ELISA Kit, c-Ros-1 ELISA Kit, v-Ros Avian UR2 Sarcoma Virus Oncogene Homolog 1 ELISA Kit, Proto-oncogene tyrosine-protein kinase ROS ELISA Kit, Receptor tyrosine kinase c-ros oncogene 1 ELISA Kit

Standard: 1000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat ROS1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat ROS1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat ROS1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat ROS1 in the samples is then determined by comparing the OD of the samples to the standard curve