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Mouse Succinate Dehydrogenase Complex Subunit C (SDHC) ELISA Kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Succinate Dehydrogenase Complex Subunit C (SDHC) .

Product Specifications

Product Name Alternative

PGL3 ELISA Kit, CYB560, SDH3 ELISA Kit, CYBL ELISA Kit, QPs1 ELISA Kit, Integral membrane protein CII-3 ELISA Kit, Succinate-ubiquinone oxidoreductase cytochrome B large subunit ELISA Kit, Succinate dehydrogenase cytochrome b560 ELISA Kit

UniProt

Q9CZB0

Reactivity

Mouse

Field of Research

Metabolism Research

Assay Type

Sandwich

Assay Performance Time

3.5h

Sample Type

Tissue homogenates and other biological fluids.

Sensitivity

0.068 ng/mL

Notes

For research use only.

Applications Notes

Standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse SDHC. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse SDHC. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse SDHC, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse SDHC in the samples is then determined by comparing the OD of the samples to the standard curve

Tested Applications

ELISA

Dynamic Range

0.16-10 ng/mL

Available Sizes

Frequently Asked Questions

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