Cattle Defensin Beta 2 (DEFb2) ELISA Kit

B-DF2 ELISA Kit, DEFB2 ELISA Kit, BD2 ELISA Kit, DEF-B2 ELISA Kit, HBD2 ELISA Kit, DEFB102 ELISA Kit, HBD-2 ELISA Kit, SAP1 ELISA Kit, DEFB4A ELISA Kit, DEFB4 ELISA Kit, Skin-Antimicrobial Peptide 1 ELISA Kit

Standard: 1000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle DEFb2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle DEFb2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle DEFb2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle DEFb2 in the samples is then determined by comparing the OD of the samples to the standard curve