Human Proteasome Subunit Alpha Type 1 (PSMa1) ELISA Kit

HC2 ELISA Kit, NU ELISA Kit, PROS30 ELISA Kit, Proteasome (Prosome, Macropain) Subunit, Alpha Type,1 ELISA Kit, Macropain subunit C2 ELISA Kit, Multicatalytic endopeptidase complex subunit C2 ELISA Kit, Proteasome nu chain ELISA Kit

Standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PSMa1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PSMa1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PSMa1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PSMa1 in the samples is then determined by comparing the OD of the samples to the standard curve