Human Dipeptidyl Peptidase 6 (DPP6) ELISA Kit

DPPX ELISA Kit, Dipeptidylpeptidase 6 ELISA Kit, Dipeptidyl aminopeptidase-like protein 6 ELISA Kit, Dipeptidyl aminopeptidase-related protein ELISA Kit, Dipeptidyl peptidase IV-like protein ELISA Kit

Standard: 200 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human DPP6. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human DPP6. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human DPP6, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human DPP6 in the samples is then determined by comparing the OD of the samples to the standard curve