Suc-AAPF-pNA

Suc-AAPF-pNA (Suc-Ala-Ala-Pro-Phe-pNA) is a chromogenic p-nitroanilide (pNA) substrate with the Km of 1.7 mM. Cleavage of Suc-AAPF-pNA releases 4-nitroaniline, which is yellow in colour and can be measured spectrophotometrically. Suc-AAPF-pNA can be used for the measurement of free and membrane-bound cathepsin G in human neutrophils[1][2][3].

Product Specifications

CAS Number

[70967-97-4]

Product Name Alternative

Suc-Ala-Ala-Pro-Phe-pNA

UNSPSC

12352209

Target

Biochemical Assay Reagents

Type

Peptides

Related Pathways

Others

Applications

COVID-19-immunoregulation

Field of Research

Others

Assay Protocol

https://www.medchemexpress.com/suc-aapf-pna.html

Purity

99.42

Solubility

DMSO : 100 mg/mL (ultrasonic)

Smiles

OC(CCC(N[C@@H](C)C(N[C@@H](C)C(N1[C@@H](CCC1)C(N[C@H](C(NC2=CC=C(C=C2)[N+]([O-])=O)=O)CC3=CC=CC=C3)=O)=O)=O)=O)=O

Molecular Formula

C30H36N6O9

Molecular Weight

624.64

References & Citations

[1]Sylvie Attucci, et al. Measurement of free and membrane-bound cathepsin G in human neutrophils using new sensitive fluorogenic substrates. Biochem J. 2002 Sep 15;366 (Pt 3) :965-70.|[2]A J Webb, et al. A protease-based biosensor for the detection of schistosome cercariae. Sci Rep. 2016 Apr 19:6:24725. |[3]Jeanette M Mucha, et al. Enhanced immunogenicity of a functional enzyme by T cell epitope modification. BMC Immunol. 2002:3:2.