Total Sulfhydryl Group/Total Thiol (-SH) Colorimetric Assay Kit
Product Specifications
Target
T-SH
Type
Colorimetric method
Field of Research
Oxidative stress
Assay Principle
Sulfhydryl compounds react with 5,5' -dithiobis (2-nitrobenzoic acid) under neutral or alkaline conditions to produce a yellow product which have a maximum absorption peak at 412 nm. Measure the OD value and calculate the total mercapto content indirectly.
Assay Performance Time
20 min
Sample Type
Serum; plasma; animal tissue
Detection Range
9.91-1000 μmol/L
Sensitivity
9.91 μmol/L
Shipping Conditions
Ice bag
Storage Conditions
This product can be stored at 2~8°C for 12 months with shading light.
Citation 01
https://www.sciencedirect.com/science/article/pii/S1319562X22004119
Inter CV
2.9%
Intra CV
2.5%
Detection Instrument
Microplate reader
Documents
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