SIAH1 Antibody
Goat polyclonal antibody to SIAH1
Product Specifications
Product Name Alternative
Anti SIAH1 antibody, anti seven in absentia homolog 1 (Drosophila) antibody, anti Siah-1 antibody, anti hSIAH1 antibody, anti HUMSIAH antibody, anti Siah-1a antibody, anti FLJ08065 antibody, anti seven in absentia homolog 1 antibody, anti sonic hedgehog homolog antibody
Reactivity
Human, Rat
Target
SIAH1
Clonality
Polyclonal
Conjugation
Unconjugated
Sequence
SRQTATALPTGTSKC
Field of Research
Cell Biology
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Dilution
Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: Approx. 37kDa band observed in Human Liver lysates and approx. 30+37kDa bands in Rat Liver lysates (calculated MW of 34.6kDa according to Human NP_001006611.1 and 31.1kDa according to Rat NP_543181.2 and 38.2kDa according to Rat XP_038953364.1) . Recommended concentration: 1-3ug/ml. Primary incubation 1 hour at room temperature. Immunofluorescence: Strong expression of the protein seen in the nucleus and cytoplasm of U2OS and HepG2 cells. Recommended concentration: 10μg/ml.
Molecular Weight
31.1; 34.6
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Prediction Reactivity
Bovine, Canine, Mouse
Tested Applications
ELISA, IF, WB
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Entrez
6477
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