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Anti-Phospho B-RAF (Thr599) Antibody

Boster Bio Anti-Phospho B-RAF (Thr599) Antibody (Catalog# P30441) . Tested in WB, IHC-P, IF (paraffin), ELISA applications. This antibody reacts with Human, Mouse, Rat.

Product Specifications

Synonyms

BRAF; BRAF1; RAFB1; Serine/threonine-protein kinase B-raf; Proto-oncogene B-Raf; p94; v-Raf murine sarcoma viral oncogene homolog B1

Gene Name

BRAF

UniProt

P15056

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

A peptide derived from human B-RAF around the phosphorylation site of Thr599. Immunogen sequence location: 571-620

Clonality

Polyclonal

Tissue Specificity

Brain and testis.

Applications

WB, IHC-P, IF (paraffin), ELISA

Purification

The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Concentration

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Form

Liquid

Function

Protein kinase involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron. Phosphorylates MAP2K1, and thereby contributes to the MAP kinase signal transduction pathway.

Molecular Weight

84437 MW

Shipping Conditions

Available

Storage Conditions

Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Fragment

Rabbit IgG

Other Gene Names

Serine/threonine-protein kinase B-raf

Subcellular Location

Nucleus. Cytoplasm. Cell membrane. Colocalizes with RGS14 and RAF1 in both the cytoplasm and membranes.

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