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STAT5A (aa681-692) Antibody

Goat polyclonal antibody to STAT5A

Product Specifications

Product Name Alternative

Anti MGF antibody, anti signal transducer and activator of transcription 5A antibody, anti STAT5 antibody, anti STAT5A antibody

Reactivity

Human

Target

STAT5A (aa681-692)

Clonality

Polyclonal

Conjugation

Unconjugated

Sequence

KYYTPVLAKAVD

Field of Research

Epigenetics

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Dilution

ELISA: 1:8000, WB: 0.1-0.3 μg/ml, IHC-P: 5 μg/ml

Molecular Weight

90.6

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

WB: Approx 100kDa band observed in lysates of cell line K562 (calculated MW of 90.6kDa according to NP_003143.2) . Recommended concentration: 0.1-0.3μg/ml. Primary incubation 1 hour at room temperature. IHC: Paraffin embedded Human Spleen. Recommended concentration: 5μg/ml.. Notes: Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371. Flow Cytometry: Flow cytometric analysis of K562 cells. Recommended concentration: 10ug/ml.

Prediction Reactivity

Bovine, Canine, Mouse, Rat

Tested Applications

ELISA, FC, IHC, WB

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

Entrez

6776

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