NP-40
Non ionic detergents like NP40 are used in immunoassays for diluting antibodies to lower background, and non-specific antigen-antibody (ag-ab) reaction.
Product Specifications
Background
Non ionic detergents like NP40 are used in immunoassays for diluting antibodies to lower background, and non-specific antigen-antibody (ag-ab) reaction. This detergent is also used in washing buffer from 0.1- 0.5% in all immunoassays (ELISA, Immunohistochemistry (IHC), Blot assays and others) to reduce background. Tergitol-type NP-40, which is nonyl phenoxylpolyethoxylethanol, lower surface tension of water.
Certification
RUO
Other Statements
For research use only; not for use in diagnostic procedures. FOR IN VITRO LABORATORY USE ONLY
Label
ICT
Type
IHC Reagents
Applications
IHC, ELISA
Assay Protocol
For diluting antibodies use 1:1000 in buffers (1 ml into 999 ml) . Washing buffer use 1:500 (1 ml into 499 ml) . The optimum dilution should be determined by the individual lab. (NOT RECOMMENDED FOR DILUTION OF S100 ANTIBODIES)
Buffer
This detergent is supplied as 25% in water with 0.15 % preservative.
Shipping Conditions
Ships overnight (domestic), International Priority Shipping
Storage Conditions
Room Temperature
Storage Temperature
Room temperature
Target Description
Non ionic detergents like NP40 are used in immunoassays for diluting antibodies to lower background, and non-specific antigen-antibody (ag-ab) reaction. This detergent is also used in washing buffer from 0.1- 0.5% in all immunoassays (ELISA, Immunohistochemistry (IHC), Blot assays and others) to reduce background. Tergitol-type NP-40, which is nonyl phenoxylpolyethoxylethanol, lower surface tension of water.
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